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Pyrosequencing Inc
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Qiagen
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Image Search Results
Journal: The Journal of Molecular Diagnostics : JMD
Article Title: Frequency and Spectrum of BRAF Mutations in a Retrospective, Single-Institution Study of 1112 Cases of Melanoma
doi: 10.1016/j.jmoldx.2012.10.002
Figure Lengend Snippet: Profile of BRAF Mutations in 499 Cutaneous Melanomas, Including Primary and Metastatic Acral and Nonacral Lesions
Article Snippet: A :
Techniques:
Journal: The Journal of Molecular Diagnostics : JMD
Article Title: Frequency and Spectrum of BRAF Mutations in a Retrospective, Single-Institution Study of 1112 Cases of Melanoma
doi: 10.1016/j.jmoldx.2012.10.002
Figure Lengend Snippet: Detection of BRAF c.1799_1800delinsAA (the so-called p.V600E2) in melanoma by pyrosequencing and Sanger sequencing. A: Pyrosequencing tracing shows the WT BRAF codon 600 as GTG. B: In mutant BRAF codon 600, a prominent peak represents A after the first G (white arrow), and a reduced peak represents the third base G (black arrow). C: The precise variant sequence (GAA) is confirmed by Sanger sequencing, which demonstrates a heterozygous pattern of T/A G/A at the second and third base positions of codon 600 (arrows).
Article Snippet: A :
Techniques: Sequencing, Mutagenesis, Variant Assay